Composite

Part:BBa_K4015013:Design

Designed by: Andrew Peng Yi   Group: iGEM21_KEYSTONE   (2021-10-21)


CotA-Lcp1VH2-HlyA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2058
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1677
    Illegal NgoMIV site found at 2035
    Illegal AgeI site found at 1348
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1662
    Illegal SapI.rc site found at 286
    Illegal SapI.rc site found at 1650


Design Notes

None


Source

The parts are assembled through Gibson assembly, and is ligated to pET23a.

References